High-resolution melting curve analysis of heat shock protein 65 for identification of mycobacterial isolates

Int J Tuberc Lung Dis. 2018 Dec 1;22(12):1511-1513. doi: 10.5588/ijtld.18.0164.

Abstract

Infections caused by non-tuberculous mycobacteria (NTM) often escape detection on traditional diagnostics. We evaluated the ability of heat shock protein 65 (hsp65) polymerase chain reaction and high-resolution melting curve (HRMC) analysis to identify and differentiate Mycobacterium tuberculosis complex (MTC) from NTM in 150 clinical mycobacterial isolates from extra-pulmonary sites. A hundred MTC and 50 NTM isolates were identified. We were able to simultaneously differentiate between seven mycobacterial species using HRMC analysis; a concordance of 100% between hsp65 HRMC analysis, polymerase chain reaction + restriction analysis and hsp65 sequencing was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques
  • Chaperonin 60 / genetics*
  • Humans
  • Mycobacterium / classification
  • Mycobacterium / genetics*
  • Mycobacterium / isolation & purification
  • Mycobacterium Infections, Nontuberculous / diagnosis*
  • Polymerase Chain Reaction

Substances

  • Bacterial Proteins
  • Chaperonin 60
  • heat-shock protein 65, Mycobacterium